MSI Tutorial - RNA-Seq Analysis

MSI Tutorial - RNA-Seq Analysis

This tutorial covers the process of handling bulk RNA-seq data with command-line tools. Attendees will learn the structure of a “standard” bulk RNA-seq analysis workflow and the names of commonly used tools to process the data. We will cover the setup and use of a command line MSI-developed analytical workflow to process a real RNA-seq dataset. Quality control metrics and basic interpretation of experiment summaries are covered. A short command line introduction is provided. Differential gene expression analysis is not covered in this tutorial.

Prerequisites: None, but Linux command line experience is beneficial

This tutorial will cover:

  • Common commands for the Linux command line
  • Basic organization of files and directories on Linux systems
  • Workflow for bulk RNA-seq data processing
  • Usage of an MSI-developed tool for bulk RNA-seq analysis
  • Common quality control metrics for bulk RNA-seq experiments

To be successful, you should have:

  • An active MSI account
  • Basic familiarity with the Linux command line
  • Knowledge of how to submit compute jobs on MSI systems
  • Basic understanding of genomics technologies

Date, time and location:

  • Mar. 26, 2024
  • 1:00pm to 3:00pm
  • 575 Walter Library and online

Click here to Register


Upcoming sessions


Tutorial Information

Recommended background: 

  • Basic understanding of gene expression

Training Level: 

  • Beginner

Tutorial format: 

  • Lecture Slides

Training Materials: 

  • Lecture Slides

  • Self-Guided PDF


  • Data Analysis


Previous Recording


Tutorial Materials

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